Ignacio Poblete Castro

Director del Laboratorio en Ingeniería en Biosistemas (bel-lab.org, BEL) donde se desarrollan las áreas de bioprocesos e ingeniería metabólica de microorganismos para la producción de químicos de interés industrial y bioconversión de compuestos recalcitrantes y tóxicos. Actualmente explotamos las capacidades metabólicas y creamos nuevas rutas biosintéticas en células bacterianas productoras de biopolímeros, biosurfactantes, nanoparticulas metálicas y carotenoides. Usamos bacterias ambientales para la remediación de diesel, PCBs, y metales pesados presentes en distintos efluentes.

Cursos

Pregrado: Diseño de Bioreactores, Ingeniería Metabólica y Bioinformática.

Postgrado: Modelado Avanzado de Bioprocesos, Tratamiento de Residuos Sólidos.

Publicaciones

  1. Y Sulbaran-Bracho, M Orellana-Saez, J Castro-Severyn, C Galbán-Malagón, E Castro-Nallar, I Poblete-Castro*. Continuous bioreactors enable high-level bioremediation of diesel-contaminated seawater at cold temperatures using Antarctic bacterial consortia: pollutant analysis and microbial community composition. Environmental pollution 321, 121139.

 

  1. Núñez-Navarro N, Poblete-Castro I, Zacconi FC, Parra LP. Discovery of New Phenylacetone Monooxygenase Variants for the Development of Substituted Indigoids through Biocatalysis. Mol. Sci. 2022, 23(20), 12544.

 

  1. Borrero de Acuña, JM, Poblete-Castro I*. Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery. Microbial Biotechnology 6 (2), 262-285.

 

  1. Bobadilla-Fazzini RA, Poblete-Castro I*. Biofilm formation is crucial for efficient copper bioleaching from bornite under mesophilic conditions: unveiling the lifestyle and catalytic role of sulfur-oxidizing bacteria. Frontiers in Microbiology. 12:761997.

 

  1. Acosta A, Antipán J, Fernández M, Prado G, Sandoval-Altamirano C, Günther G, Gutiérrez-Urrutia I, Poblete-Castro I, Vega A, Pizarro N. Photochemistry of P, N-bidentate rhenium (i) tricarbonyl complexes: reactive species generation and potential application for antibacterial photodynamic therapy. RSC Advances 11:31959–31966.

 

  1. Hoffmann, M. Kohlstedt, L. Jungmann, M. Hutter, I. Poblete-Castro, J. Becker, C. Wittmann. Cascaded valorization of brown seaweed to produce l-lysine and value-added products using Corynebacterium glutamicum streamlined by systems metabolic engineering. 2021. Metabolic Engineering. 67, 297-307.

 

  1. Borrero de Acuña, M. Rhode, C. Saldias,I Poblete-Castro*. Fed-batch mcl-Polyhydroxyalkanoates production in Pseudomonas putida KT2440 and ΔphaZ mutant on biodiesel-derived crude glycerol. 2021. Frontiers in Bioengineering and Biotechnology 9, 642023.

 

  1. I Poblete-Castro, SL Hoffmann, J Becker, C Wittmann. Cascaded valorization of seaweed using microbial cell factories. Current Opinion in Biotechnology 65, 102-113.

 

  1. JM Borrero‐de Acuña, I Gutierrez‐Urrutia, C Hidalgo‐Dumont, C Aravena‐Carrasco, M Orellana‐Saez, N Palominos‐Gonzalez, JBJH van Duuren, V Wagner, L Gläser, J Becker, M Kohlstedt, FC Zacconi, C Wittmann, I Poblete‐Castro*. Channeling carbon flux through the meta‐cleavage route for improved poly (3‐hydroxyalkanoate) production from benzoate and lignin‐based aromatics in Pseudomonas putidaH 2020. Microbial Biotechnology.

 

  1. JM Borrero-de Acuña, I Poblete-Castro*. Expanding the reach of recombineering to environmental bacteria. 2020. Trends in Biotechnology 38 (7), 684-685.

 

  1. I Poblete-Castro, C Aravena-Carrasco, M Orellana-Saez, N Pacheco, A Cabrera, JM. Borrero-de Acuña. Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates). 2020. Frontiers in Bioengineering and Biotechnology8, 161.

 

  1. I Poblete-Castro, C Wittmann, PI. Nikel. Biochemistry, genetics and biotechnology of glycerol utilization in Pseudomonas species. 2020. Microbial Biotechnology13 (1), 32-53.

 

  1. van Duuren, J. B., de Wild, P., Starck, S., Bradtmöller, C., Selzer, M., Mehlmann, K., Schneider, R., Kohlstedt, M., Poblete-Castro, I., Stolzenberger, J., Barton, N., Fritz, M., Scholl, S., Venus, J. & Wittmann, C. Limited life cycle and cost assessment for the bioconversion of lignin‐derived aromatics into adipic acid. 2020. Biotechnology and Bioengineering. 117 (5), 1381-1393.

 

  1. T Godard, D Zühlke, G Richter, M Wall, M Rohde, K Riedel, I Poblete-Castro, R Krull, R Biedendieck. Metabolic Rearrangements Causing Elevated Proline and Polyhydroxybutyrate Accumulation During the Osmotic Adaptation Response of Bacillus megaterium. 2020. Frontiers in Bioengineering and Biotechnology8, 47.

 

  1. C Pardo-Esté, J Castro-Severyn, GI. Krüger, CE. Cabezas, AC. Briones, C Aguirre, N Morales, MS Baquedano, YN Sulbaran-Bracho, AA Hidalgo, C Meneses, I Poblete-Castro, E Castro-Nallar, MA Valvano and CP Saavedra. The transcription factor ArcA modulates Salmonella’s metabolism in response to neutrophils hypochlorous acid-mediated stress. 2019. Frontiers in Microbiology10, 2774. 

 

  1. M Orellana-Saez, N Pacheco, JI Costa, KN Mendez, MJ Miossec, C Meneses, E Castro-Nallar, AE Marcoleta, I Poblete-Castro*. In-depth genomic and phenotypic characterization of the Antarctic psychrotolerant strain Pseudomonas sp. MPC6 reveals unique metabolic features, plasticity, and biotechnological potential. Frontiers in Microbiology 10, 1154. 

 

  1. N Pacheco, M Orellana-Saez, M Pepczynska, J Enrione, M Bassas-Galia, JM Borrero-de Acuña, FC Zacconi, AE Marcoleta, I Poblete-Castro*. Exploiting the natural poly (3-hydroxyalkanoates) production capacity of Antarctic Pseudomonas strains: from unique phenotypes to novel biopolymers.Journal of industrial Microbiology & Biotechnology 46 (8), 1139-1153. 

 

  1. JM Borrero-de Acuña, C Aravena-Carrasco, I Gutierrez-Urrutia, D Duchens, I Poblete-Castro*. Enhanced synthesis of medium-chain-length Poly (3-hydroxyalkanoates) by inactivating the tricarboxylate transport system of Pseudomonas putidaKT2440 and process development using waste vegetable oil.  Process Biochemistry 77, 23-30. 

 

  1. I Gutierrez-Urrutia, MJ Miossec, SL Valenzuela, C Meneses, VAP Martins dos Santos, E Castro-Nallar, I Poblete-Castro*. Genome sequence of two members of the chloroaromatic-degrading MT community: Pseudomonas reinekei MT1 and Achromobacter xylosoxidans 2018. Journal of Biotechnology 275, 13-16. 

 

  1. Oliva-Arancibia B, Órdenes-Aenishanslins N, Bruna N, Ibarra PS, Zacconi FC, Pérez-Donoso JM, Poblete-Castro I.* Co-synthesis of medium-chain-length polyhydroxyalkanoates and CdS quantum dots nanoparticles in Pseudomonas putida2017. Journal of Biotechnology 264, 29-37. 

 

  1. JM Borrero-de Acuña, C Hidalgo-Dumont, N Pacheco, A Cabrera, I Poblete-Castro. A novel programable lysozyme-based lysis system in Pseudomonas putidafor biopolymer production.  Scientific Report 7 (1), 4373. 

 

  1. CE Torres-Cerna, AY Alanis, I Poblete-Castro, EA Hernandez-Vargas. Batch cultivation model for biopolymer production. Chemical and Biochemical Engineering Quarterly 31, 8. 

 

  1. M Varas, C Valdivieso, C Mauriaca, J Ortíz-Severín, A Paradela, I Poblete-Castro, R Cabrera, FP Chávez. Multi-level evaluation of Escherichia colipolyphosphate related mutants using global transcriptomic, proteomic, and phenomic analysis. 2017. Biochimica et Biophysica Acta (BBA)-General Subjects 4, 871-883. 

 

  1. Beckers V, Poblete-Castro I, Tomasch J, Wittmann C.Integrated analysis of gene expression and metabolic fluxes of PHA-producing Pseudmonas putida grown on glycerol.  Microbial Cell Factories 15, 73. 

 

  1. Vizoso P, Pacheco N, Bastias-Molina M, Meneses C, Poblete-Castro I*.Draft Genome Sequence of the Phenol-Degrading Bacterium Pseudomonas putida 2015. Genome Announcement 3, 4. 

 

  1. Poblete-Castro I*,Binger D, Oehlert R, Rohde M. Comparison of mcl-Poly(3-hydroxyalkanoates) synthesis by different Pseudomonas putida strains from crude glycerol: citrate accumulates at high titer under PHA-producing conditions.  BMC Biotechnology 14, 962.

 

  1. Borrero-de Acuña JM, Bielecka A, Häussler S, Schobert M, Jahn M, Wittmann C, Jahn D, Poblete-Castro I*. Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida. Microbial Cell Factories 13, 88.

 

  1. Poblete-Castro I*, Rodriguez A, Lam, C, Kessler W. Improved production of medium-chain-length Polyhydroxyalkanotes in glucose-based fed-batch cultivations of metabolically engineered Pseudomonas putida2014. Journal of Microbiology and Biotechnology 24 (1), 59-69.

 

  1. Poblete-Castro I, Binger D, Rodriguez A, Becker J, dos Santos VAP, Wittmann C. In-silico driven metabolic engineering of Pseudomonas putida for the enhanced production of polyhydroxyalkanoates. Metabolic Engineering 15, 113-123.

 

  1. Poblete-Castro I*, Escapa I, Jager C, Puchalka J, Lam C, Shomburg D, Prieto MA, dos Santos VAP. The metabolic response of Pseudomonas putida KT2442 producing high levels of polyhydroxyalkanoates under single- and multiple-nutrient-limited growth: Highlights from a multi- level omics approach. Microbial Cell Factories 11, 34.

 

  1. Poblete-Castro I, Becker J, Dohnt K, dos Santos V, Wittmann C. Industrial biotechnology of Pseudomonas putida and related species. Applied Microbiology and Biotechnology 93(6), 2279- 2290.

Proyectos

2021-2024           Proyecto Fondecyt Regular ANID. Turning plastic into bioplastic: Engineering Pseudomonas strains for the efficient synthesis of Polyhydroxyalkanoates from PET derived compounds. Investigador Principal.

2021-2024           Proyecto Fondecyt Regular ANID. Biopolymers production from solid urban wastes. Co-investigador.

2020-2023           Proyecto Anillo-ANID-PIA ACT192057. Long-range transport of xenobiotics and microorganisms: teleconnections and influence on terrestrial ecosystems. Subdirector.

2021-2022           Proyecto INACH RG_17_19. A systems-wide understanding of the Antarctic Pseudomonas frigusceleri MPC6 strain for fast-growing and novel biopolymer synthesis at low temperatures. Investigador Principal.

2017-2020           System-level analysis of Arabidopsis thaliana Pseudomonas syringae interaction: dual assessment of the transcriptomic and metabolomic responses to unravel bacterial effects leading to resistance or susceptibility through the regulatory node WRKY7/11/17, FONDECYT, Co-investigador.

2016-2019            LignoValue. BMBF. Germany. Co-investigador

2016- 2018           Systems metabolic engineering of Pseudomonas putida for the synthesis of tailor-made polyhydroxyalkanoates, FONDECYT. Investigador    Principal

Libros y/o Capítulos

  • Host organism: Pseudomonas putida. I Poblete-Castro, JM Borrero-de Acuña, PI Nikel, M Kohlstedt and C Wittmann. Industrial Biotechnology: Microorganisms. Wiley-Blackwell Biotechnology Book Series. 2017.

Títulos y/o Grados

  • Doctor en Biotecnología. Technische Universitat Braunschweig, Alemania.
  • Magister en Medio Ambiente. Universidad De Santiago de Chile.
  • Ingeniero Ambiental. Universidad Tecnológica Metropolitana, Chile

Líneas de Investigación

  • Ingeniería de Bioprocesos
  • Ingeniería Metabólica
  • Biotecnología Microbiana de Sistemas